Lts shown in Figure 8A demonstrated a large increase in [Ca

Lts shown in Figure 8A demonstrated a large increase in [Ca2+]i due to A23187 as expect without any significant difference between undifferentiated vs. differentiated cells. CG (16 ppm) caused significant decreases (pStaurosporine expression is predominantly localized to the neurite body, whereas synapsin-1/2 accumulates within the growth cone.Andres et al. BMC Neuroscience 2013, 14:49 http://www.biomedcentral.com/1471-2202/14/Page 7 ofFigure 4 Expression of Neuron Specific Proteins in M17 cell cultures. M17 neuroblastoma cells were grown and treated with or without 10 M RA for 72 hours to induce differentiation. The cells were washed and solubilized in sample buffer and analyzed by Western blotting for (A) SNAP-25, (B) synapsin, and (C) vimentin. Either -actin or GAPDH was used as a house keeping protein marker to show equal protein loading of gels. The relative amount of each marker protein was quantified by densitometric analysis using Image J program (NIH public domain program, http://rsbweb. nih.gov/ij/index.html). Differences in marker proteins in differentiated vs. undifferentiated cells were assessed in terms of normalized optical density in differentiated cells compared to undifferentiated (no RA) controls as shown in the bottom panel (D). Normalized optical density of undifferentiated control was 100 for each type of marker protein. **pPubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/16989806 a house keeping protein marker to show equal protein loading of gels. The relative amount of each marker protein was quantified by densitometric analysis using Image J program (NIH public domain program, http://rsbweb. nih.gov/ij/index.html). Differences in marker proteins in differentiated vs. undifferentiated cells were assessed in terms of normalized optical density in differentiated cells compared to undifferentiated (no RA) controls as shown in the bottom panel (D). Normalized optical density of undifferentiated control was 100 for each type of marker protein. ND = not detected and as such could not be quantified. For M1 mAcR, the difference between undifferentiated vs differentiated was not statistically significant (student t test). n=4.which positive compounds would be selected for in vivo evaluation. Prior studies using in vitro cellular.